rat anti-mouse pmn Search Results


pmn  (Bio-Rad)
96
Bio-Rad pmn
( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with <t>PMN,</t> CD3, GFAP, NeuN, <t>and</t> <t>GST-π.</t> ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Pmn, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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mouse anti pmn fcriii cd 16 mab  (Bio-Rad)
94
Bio-Rad mouse anti pmn fcriii cd 16 mab
( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with <t>PMN,</t> CD3, GFAP, NeuN, <t>and</t> <t>GST-π.</t> ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Mouse Anti Pmn Fcriii Cd 16 Mab, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti pmn antibody ab  (Cedarlane)
93
Cedarlane rabbit anti pmn antibody ab
( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with <t>PMN,</t> CD3, GFAP, NeuN, <t>and</t> <t>GST-π.</t> ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Rabbit Anti Pmn Antibody Ab, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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mouse ly6g anti pmn  (Bio-Rad)
93
Bio-Rad mouse ly6g anti pmn
( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with <t>PMN,</t> CD3, GFAP, NeuN, <t>and</t> <t>GST-π.</t> ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Mouse Ly6g Anti Pmn, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti-mouse+pmn/Rat+anti+Mouse+Ly-6G/pmc05203657-404-21-29
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polyclonal rabbit anti rat pmn antibody  (Cedarlane)
93
Cedarlane polyclonal rabbit anti rat pmn antibody
( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with <t>PMN,</t> CD3, GFAP, NeuN, <t>and</t> <t>GST-π.</t> ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Polyclonal Rabbit Anti Rat Pmn Antibody, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti-mouse+pmn/Anti-Rat+PMN%2C+Adsorbed+Antiserum%2C+(Polyclonal)+(rabbit+serum)/10__1161_slash_01__atv__0000237605__25666__13-68-21-28
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rabbit anti pmn accurate antibodies antibodies  (Vector Laboratories)
96
Vector Laboratories rabbit anti pmn accurate antibodies antibodies
( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with <t>PMN,</t> CD3, GFAP, NeuN, <t>and</t> <t>GST-π.</t> ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Rabbit Anti Pmn Accurate Antibodies Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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rat anti mouse pmn  (Bio-Rad)
96
Bio-Rad rat anti mouse pmn
( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with <t>PMN,</t> CD3, GFAP, NeuN, <t>and</t> <t>GST-π.</t> ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Rat Anti Mouse Pmn, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti-mouse+pmn/Rat+anti+Mouse+CD68/pmc03133655-104-8-13
Average 96 stars, based on 1 article reviews
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biotinylated rat anti-mouse gr-1  (Becton Dickinson)
90
Becton Dickinson biotinylated rat anti-mouse gr-1
( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with <t>PMN,</t> CD3, GFAP, NeuN, <t>and</t> <t>GST-π.</t> ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Biotinylated Rat Anti Mouse Gr 1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat anti mouse monoclonal antibody  (Bio-Rad)
93
Bio-Rad rat anti mouse monoclonal antibody
Figure 8. Immunohistochemistry failed to detect renal T cell recruitment after LPS administration. Spleen tissue sections from untreated control mice were used as positive controls to test both <t>monoclonal</t> antibodies (a; only staining with clone CD3-12 is shown). Independent of the antibody used, we could detect, however, only a few random CD3 cells (arrows) in kidneys that were harvested before (b), 4 h after (c), or 24 h after (d) LPS administration. These cells were either nearby or inside larger vessels. Thus, LPS did not seem to induce a substantial renal T cell recruitment. Hematoxylin counterstain. Magnifica- tion, 10 in left column, 40 in right column.
Rat Anti Mouse Monoclonal Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat anti mouse f4 80  (Bio-Rad)
96
Bio-Rad rat anti mouse f4 80
Figure 8. Immunohistochemistry failed to detect renal T cell recruitment after LPS administration. Spleen tissue sections from untreated control mice were used as positive controls to test both <t>monoclonal</t> antibodies (a; only staining with clone CD3-12 is shown). Independent of the antibody used, we could detect, however, only a few random CD3 cells (arrows) in kidneys that were harvested before (b), 4 h after (c), or 24 h after (d) LPS administration. These cells were either nearby or inside larger vessels. Thus, LPS did not seem to induce a substantial renal T cell recruitment. Hematoxylin counterstain. Magnifica- tion, 10 in left column, 40 in right column.
Rat Anti Mouse F4 80, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fitc-conjugated rat-anti-mouse macrophage/monocyte antibody moma-2  (ImmunoKontact)
90
ImmunoKontact fitc-conjugated rat-anti-mouse macrophage/monocyte antibody moma-2
Figure 8. Immunohistochemistry failed to detect renal T cell recruitment after LPS administration. Spleen tissue sections from untreated control mice were used as positive controls to test both <t>monoclonal</t> antibodies (a; only staining with clone CD3-12 is shown). Independent of the antibody used, we could detect, however, only a few random CD3 cells (arrows) in kidneys that were harvested before (b), 4 h after (c), or 24 h after (d) LPS administration. These cells were either nearby or inside larger vessels. Thus, LPS did not seem to induce a substantial renal T cell recruitment. Hematoxylin counterstain. Magnifica- tion, 10 in left column, 40 in right column.
Fitc Conjugated Rat Anti Mouse Macrophage/Monocyte Antibody Moma 2, supplied by ImmunoKontact, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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r-phycoerythrin-conjugated rat anti-mouse ly-6g (gr1; cd11b  (Becton Dickinson)
90
Becton Dickinson r-phycoerythrin-conjugated rat anti-mouse ly-6g (gr1; cd11b
Figure 8. Immunohistochemistry failed to detect renal T cell recruitment after LPS administration. Spleen tissue sections from untreated control mice were used as positive controls to test both <t>monoclonal</t> antibodies (a; only staining with clone CD3-12 is shown). Independent of the antibody used, we could detect, however, only a few random CD3 cells (arrows) in kidneys that were harvested before (b), 4 h after (c), or 24 h after (d) LPS administration. These cells were either nearby or inside larger vessels. Thus, LPS did not seem to induce a substantial renal T cell recruitment. Hematoxylin counterstain. Magnifica- tion, 10 in left column, 40 in right column.
R Phycoerythrin Conjugated Rat Anti Mouse Ly 6g (Gr1; Cd11b, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with PMN, CD3, GFAP, NeuN, and GST-π. ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Journal: Science Advances

Article Title: Macrophage centripetal migration drives spontaneous healing process after spinal cord injury

doi: 10.1126/sciadv.aav5086

Figure Lengend Snippet: ( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with PMN, CD3, GFAP, NeuN, and GST-π. ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: The following primary antibodies were used: CD68 (rat, MCA1957; 1:200; Serotec), GFAP (rabbit, Z0334; 1:200; Dako), IRF8 (goat, sc-6058; 1:200; Santa Cruz Biotechnology), GST-π (mouse, no. 610719; 1:200; BD Biosciences), NeuN (mouse, MAB377; 1:200; Chemicon), PMN (rat, MCA771GA; 1:200; Serotec), CD3 (145-2C11; 1:200; eBioscience), 5-HT (goat, no.20079; 1:200; ImmunoStar), platelet-derived growth factor receptor-β (PDGFRβ) (rat, no.136002; 1:200; BioLegend), and C5a (rabbit, BS-0324R; 1:200; Bioss).

Techniques: Staining, Expressing, Fluorescence, Double Immunostaining, Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Isolation

Figure 8. Immunohistochemistry failed to detect renal T cell recruitment after LPS administration. Spleen tissue sections from untreated control mice were used as positive controls to test both monoclonal antibodies (a; only staining with clone CD3-12 is shown). Independent of the antibody used, we could detect, however, only a few random CD3 cells (arrows) in kidneys that were harvested before (b), 4 h after (c), or 24 h after (d) LPS administration. These cells were either nearby or inside larger vessels. Thus, LPS did not seem to induce a substantial renal T cell recruitment. Hematoxylin counterstain. Magnifica- tion, 10 in left column, 40 in right column.

Journal: Journal of the American Society of Nephrology

Article Title: T Cells Modulate Neutrophil-Dependent Acute Renal Failure during Endotoxemia: Critical Role for CD28

doi: 10.1681/asn.2004050381

Figure Lengend Snippet: Figure 8. Immunohistochemistry failed to detect renal T cell recruitment after LPS administration. Spleen tissue sections from untreated control mice were used as positive controls to test both monoclonal antibodies (a; only staining with clone CD3-12 is shown). Independent of the antibody used, we could detect, however, only a few random CD3 cells (arrows) in kidneys that were harvested before (b), 4 h after (c), or 24 h after (d) LPS administration. These cells were either nearby or inside larger vessels. Thus, LPS did not seem to induce a substantial renal T cell recruitment. Hematoxylin counterstain. Magnifica- tion, 10 in left column, 40 in right column.

Article Snippet: As described previously (28), paraffin-embedded kidney sections (5 m) were incubated with a rat anti-mouse monoclonal antibody (clone 7/4; Serotec, Dusseldorf, Germany) against a polymorphic 40-kD antigen expressed by PMN.

Techniques: Immunohistochemistry, Control, Bioprocessing, Staining